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Forced Suppression of let-7a-5p in Mouse Blastocysts Improves Implantation Rate

Jalleh Shakerzadeh, Mansoureh Movahedin, Akram Eidi, Nasim Hayati Roodbari, Kazem Parivar
Journal Papers , 2021 February 1, {Pages }

Abstract

BackgroundEmbryo implantation requires appropriate communication between the blastocyst and endometrium. Recurrent implantation failure is an essential component of assisted reproductive technology. Also, miRNAs-mediated gene expression impacts the implantation process, and the down-regulation of some miRs, such as mmu-let-7a, improves this process. In the present study, we evaluated the effect of let-7a forced-suppression on the mouse implantation rate.

Correlation between the Energy Density of Ultraviolet Radiation and Skin Thickening In an Animal Mouse Model: Induction of Trichoepithelioma in a Mouse Model

Mohadeshe Estaji, Manijhe Mokhtari-Dizaji, Mansoureh Movahedin, Sahar Ghaffari Khaligh
Journal PapersIranian Journal of Medical Physics , Volume 18 , Issue 2, 2021 March , {Pages 106-110 }

Abstract

Ultraviolet (UV) radiation causes direct and indirect damage to the cell structure, thereby increasing the aging process. Ultraviolet B-rays (UVB)(with 280-320 nm wavelengths) cause direct damage to the skin, and the portion that reaches the epidermal cells induces biological damage [1] and the most important biological damage is inflicted by DNA damage. With UVB absorption, thymine bases in the DNA bind to adjacent thymine [2]. This evidence can affect protein synthesis. The accumulation of unrepaired damages can disrupt the cell cycle and create cell death. Moreover, these damages can sometimes destroy the cell apoptotic ability and increase the malignancies. The results depend on the type of tissue, UV dose, and its wavelength [3]. UV ra

The Antioxidant Effects of Calligonum Extract on Oxidative Stress in Spermatogonial Stem Cells Culture

Shirin Barati, Mansoureh Movahedin
Journal PapersPharmaceutical Sciences , 2021 April 24, {Pages }

Abstract

Background: Spermatogenesis is a programmed route for germ cell proliferation and differentiation that can produce abundant numbers of spermatozoa. The antioxidants play a vital role in decreasing oxidative stress production in cells; therefore, the extraction of plants with antioxidant property can prevent cell damage. In the present study, antioxidant effects of Calligonum extract on proliferation and colonization rate of spermatogonial cells was assessed.Materials and Methods: After isolation and culturing of spermatogonial stem cells (SSCs) on neonatal mice (4-5 days old) and identification by PLZF and Oct4 markers, the therapeutic effect of Calligonum comosum extract on cells treated with H2O2 was measured. The cultured cells were divi

Comparison of two methods for prolong storage of decellularized mouse whole testis for tissue engineering application: An experimental study

Nasrin Majidi Gharenaz, Mansoureh Movahedin
Journal PapersInternational Journal of Reproductive BioMedicine , Volume 19 , Issue 4, 2021 April 10, {Pages 321-332 }

Abstract

Background: Biological scaffolds are derived by the decellularization of tissues or organs. Various biological scaffolds, such as scaffolds for the liver, lung, esophagus, dermis, and human testicles, have been produced. Their application in tissue engineering has created the need for cryopreservation processes to store these scaffolds.Objective: The aim was to compare the two methods for prolong storage testicular scaffolds.Materials and Methods: In this experimental study, 20 male NMRI mice (8 wk) were sacrificed and their testes were removed and treated with 0.5% sodium dodecyl sulfate followed by Triton X-100 0.5%. The efficiency of decellularization was determined by histology and DNA quantification. Testicular scaffolds were stored in

El cultivo in vitro en test?culo de rat?n de c?lulas madre espermatogoniales humanas sostiene la progresi?n de la espermatog?nesis de forma similar al trasplante in vivo

M Mohaqiq, M Movahedin, Z Mazaheri, N Amirjannati
Journal PapersRevista Internacional de Androlog?a , Volume 19 , Issue 2, 2021 January , {Pages 112-122 }

Abstract

ObjetivoLa introducci?n de sistemas alternativos in vivo es muy importante para los pacientes con c?ncer que son tratados con tratamiento gonadot?xico. En este estudio, examinamos la progresi?n del proceso de espermatog?nesis despu?s del trasplante de c?lulas madre espermatogoniales en condiciones de cultivo de tejidos in vivo. Materiales y m?todosSe obtuvieron c?lulas madre espermatogoniales humanas a partir de una muestra de extracciones de esperma testicular y se confirm? la caracterizaci?n de estas c?lulas mediante la detecci?n de la prote?na de dedo de cinc con leucemia promieloc?tica. Estas c?lulas, despu?s de marcarse con di-alquil indocarbocianina (DiI), se trasplantaron a test?culos de rat?n con azoospermia adulta, tratados con bus

Alterations of miR-16, miR-let-7a and their target genes expression in human blastocysts following vitrification and re-vitrification

M Daneshvar, M Movahedin, M Salehi, M Noruzinia
Journal Papers , , {Pages }

Abstract

Formation of organoid-like structures in the decellularized rat testis

M Kiani, M Movahedin, I Halvaei, M Soleimani
Journal Papers , , {Pages }

Abstract

تعیین مؤلفه‌های بهینه امواج فراآوایی بر روند اسپرم‌زایی بیضه موش نوزاد (مقاله پژوهشی)‎

محمدی, شقایق, موحدین, مختاری دیزجی‎
Journal Papers , , {Pages }

Abstract

Determination of optimal parameters of ultrasonic waves on permatogenesis process of neonate mouse testis

S Mohamadi, M Movahedin, M Mokhtari Dizaji
Journal Papers , , {Pages }

Abstract

Attenuation of aquaporin‐3 may be contributing to low sperm motility and is associated with activated caspase‐3 in asthenozoospermic individuals

P Mohammadi, SA Mesbah‐Namin, M Movahedin
Journal Papers , , {Pages }

Abstract

نگه داری طولانی مدت داربست بیضه ای برای استفاده در مهندسی بافت‎

N Majidi Gharenaz, M Movahedin
Journal Papers , , {Pages }

Abstract

Numerical Study on the Complete Separation of Blood Cells using the Integrated Dielectrophoretic-Photophoretic Method in a New Microchannel

O Zahedi Siani, M Zabetian Toroghi, M Sojoodi, M Movahedin
Journal Papers , , {Pages }

Abstract

Caracterización de las células madre embrionarias derivadas de células madre espermatogoniales de ratón, después del tratamiento con ultrasonido de baja intensidad

A Azimi, M Mohaqiq, M Movahedin, Z Mazaheri
Journal Papers , , {Pages }

Abstract

The Epigenetic Assessment of Human Spermatogenic Cells Derived from Obstructive Azoospermic Patients in Different Culture Systems

M Zahiri, M Movahedin, SJ Mowla, M Noruzinia, M Koruji
Journal Papers , , {Pages }

Abstract

El cultivo in vitro en testículo de ratón de células madre espermatogoniales humanas sostiene la progresión de la espermatogénesis de forma similar al trasplante in vivo

M Mohaqiq, M Movahedin, Z Mazaheri, N Amirjannati
Journal Papers , , {Pages }

Abstract

Three-Dimensional Culture of Mouse Spermatogonial Stem Cells Using A Decellularised Testicular Scaffold.

Nasrin Majidi Gharenaz, Mansoureh Movahedin, Zohreh Mazaheri
Journal PapersCell Journal (Yakhteh) , Volume 21 , Issue 4, 2020 January 1, {Pages }

Abstract

Objective: Applications of biological scaffolds for regenerative medicine are increasing. Such scaffolds improve cell attachment, migration, proliferation and differentiation. In the current study decellularised mouse whole testis was used as a natural 3 dimensional (3D) scaffold for culturing spermatogonial stem cells.Materials and Methods: In this experimental study, adult mouse whole testes were decellularised using sodium dodecyl sulfate (SDS) and Triton X-100. The efficiency of decellularisation was determined by histology and DNA quantification. Masson’s trichrome staining, alcian blue staining, and immunohistochemistry (IHC) were done for validation of extracellular matrix (ECM) proteins. These scaffolds were recellularised through

Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study.

O Siani Zahedi, Mahdi Sojoodi, M Targhi Zabetian, MANSOUREH Movahedin
Journal PapersCell journal , Volume 22 , Issue 2, 2020 July , {Pages 218-226 }

Abstract

Objective: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the application of only a one-stage separation process. Materials and Methods: In this numerical study, we based the separation process on the differences in the particle sizes. We used the various negative DEP forces on the particles caused by the electrodes to separate them with a high efficiency. The particle separator could separate blood cells because of their different sizes. Results: Blood cells greater than 12 μm were guided to a special branch, which

Differential gene expression by lithium chloride induction of adipose-derived stem cells into neural phenotype cells

Samaneh Farrokhfar, Taki Tiraihi, Mansoureh Movahedin, Hossein Azizi
Journal PapersIranian Journal of Basic Medical Sciences , Volume 23 , Issue 4, 2020 April 1, {Pages 544-550 }

Abstract

Objective(s): Adipose-derived stem cells (ADSCs), with suitable and easy access, are multipotential cells that have the ability for differentiation into other mesodermal and transdifferentiate into neural phenotype cells. In this study, Lithium chloride (LiCl) was used for in vitro transdifferentiation of rat ADSCs into neuron-like cells (NLCs).Materials and Methods: ADSCs were isolated from the rats’ perinephric region using Dulbecco΄s Modified Eagle΄s Medium (DMEM) with Fetal Bovine Serum (FBS), cultured for 3 passages, characterized by flowcytometry and differentiation into adipogenic and osteogenic phenotypes. The ADSCs were exposed to 0.1, 0.5, 1, 1.5, 2, 5, and 10 millimolar (mM) LiCl without serum for 24 hr. The optimum dose of L

Dielectrophoretic separation of monocytes from cancer cells in a microfluidic chip using electrode pitch optimization

Omid Zahedi Siani, Mohammad Zabetian Targhi, Mahdi Sojoodi, Mansoureh Movahedin
Journal PapersBioprocess and Biosystems Engineering , 2020 April 23, {Pages 14-Jan }

Abstract

This study proposes a microfluidic device capable of separating monocytes from a type of cancer cell that is called T-cell acute lymphoblastic leukemia (RPMI-8402) in a continuous flow using negative and positive dielectrophoretic forces. The use of both the hydrodynamic and dielectrophoretic forces allows the separation of RPMI-8402 from monocytes based on differences in their intrinsic electrical properties and sizes. The specific crossover frequencies of monocytes and RPMI-8402 cells have been obtained experimentally. The optimum ranges of electrode pitch-to-channel height ratio at the cross sections with different electrode widths have been generally calculated by numerical simulations of the gradients of the electric field intensities

Correlation of energy density of ultraviolet radiation with skin thickening in an animal mouse model: Creation of a Trichoepithelioma model

Manijhe Mokhtari-Dizaji, Mohadeshe Estaji, Mansoreh Movahedin, Sahar Ghaffari Khaligh
Journal PapersIranian Journal of Medical Physics , 2020 March 16, {Pages }

Abstract

Introduction: Ultraviolet (UV) radiation to the skin causes oxidative damage and causes cancer in the epidermis. There seems to be a relationship between the thickening of the skin layer and the carcinogenicity. The aim of this study was to establish a rare benign skin lesion of Trichoepithelioma in an animal model and to investigate the relationship between the radiation dose of ultraviolet waves with thickening of the skin layer resulting from high-frequency ultrasound images.Materials and Methods: To investigate the skin damage process, 25 C57BL6 mice were irradiated with UVB (5 times a week for 9 weeks) with an energy density of 135, 270, 405, 540, 675, 810, 945, 1080 and 1215 J/m2, from the first week to the ninth week, respectively. T

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