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Vitamin D Changes Expression of DNA Repair Genes in the Patients with Multiple Sclerosis

Roya Amirinejad, Zeinab Shirvani-Farsani, Bahar Naghavi Gargari, Mohhamad Ali Sahraian, Bahram Mohammad Soltani, Mehrdad Behmanesh
Journal PapersGene , 2021 February 13, {Pages 145488 }

Abstract

Oxidative stress (OS) plays an essential role in demyelination and tissue injury related to pathogenesis of multiple sclerosis (MS). On the other hand, vitamin D (VD) as an antioxidant reduces oxidative stress and has been used as adjuvant therapy in autoimmune diseases. Although VD supplementation is suggested as a protective and immunomodulation factor for MS patients, the molecular mechanisms remain unclear. Given that VD may modulate the immune system of MS patients through the DNA repair pathway, we aimed to evaluate the effects of VD supplementation in DNA repair genes expression including OGG1, MYH, MTH1, and ITPA. Transcript levels were measured using the RT-qPCR method in peripheral blood mononuclear cells (PBMCs) of relapsing-remi

TGF-β Isoforms and Receptors: A Gene Expression Analysis in Multiple Sclerosis Patients and Normal Individuals

Zeinab Shirvani-Farsani, Mehrdad Behmanesh, Mohammad Ali Sahraian
Journal Papers , 2021 January 19, {Pages }

Abstract

Objective: Multiple sclerosis (MS) is a chronic in ammatory disease of the central nervous system (CNS), depicted by lymphocytic in ltration and demyelination. MS is associated with the up-regulation of proin ammatory and down-regulation of anti-in ammatory cytokines. The purpose of this experimental study was to evaluate the expression level of TGF-β1, TGF-β 2, TGF-β-R1 and TGF-β-R2 mRNAs in peripheral blood mononuclear cells (PBMCs) from MS patients and healthy controls using Real-Time PCR.Results: Our ndings indicated that the TGF-β-R1 expression level was 2.25 times higher in controls than MS patients. Also, a signi cant correlation between normalized expression of TGF-β-R1 and TGF-β1, or TGF-β2 was observed. Therefore, these ge

Diversity of microbes colonizing forages of varying lignocellulose properties in the sheep rumen

Mohammad Farhad Vahidi, Javad Gharechahi, Mehrdad Behmanesh, Xue-Zhi Ding, Jian-Lin Han, Ghasem Hosseini Salekdeh
Journal PapersPeerJ , Volume 9 , 2021 January 11, {Pages e10463 }

Abstract

Background The rumen microbiota contributes strongly to the degradation of ingested plant materials. There is limited knowledge about the diversity of taxa involved in the breakdown of lignocellulosic biomasses with varying chemical compositions in the rumen. Method We aimed to assess how and to what extent the physicochemical properties of forages influence the colonization and digestion by rumen microbiota. This was achieved by placing nylon bags filled with candidate materials in the rumen of fistulated sheep for a period of up to 96 h, followed by measuring forage’s chemical characteristics and community structure of biofilm-embedded microbiota.

Fast fluorescent screening assay and dual electrochemical sensing of bacterial infection agent (Streptococcus agalactiae) based on a fluorescent-immune nanofibers

R Ghasemi, SZ Mirahmadi-zare, A Allafchian, M Behmanesh
Journal Papers , , {Pages }

Abstract

Fingolimod Regulates BDNF-AS and HOTAIR Expression in Multiple Sclerosis Patients

MA Sahraian, M Javan, M Behmanesh
Journal Papers , , {Pages }

Abstract

Expression of remyelination-modulating genes in astrocytes is regulated by MALAT1 and Lnc-DC long non-coding RNAs

S Askari, M Behmanesh
Journal Papers , , {Pages }

Abstract

Hotair and Malat1 Long Noncoding RNAs regulate Bdnf Expression and Oligodendrocyte Precursor Cells Differentiation

L Zare, MA Sahraian, M Javan, M Behmanesh
Journal Papers , , {Pages }

Abstract

Fingolimod Regulates BDNF-AS and HOTAIR Expression in Multiple Sclerosis Patients

F khani Habibabadi, MA Sahraian, M Javan, M Behmanesh
Journal Papers , , {Pages }

Abstract

SNHG7 has an oncogenic role in colorectal cancer via potential sponging of MIR-485-5P and MIR-193A-5P; in silico approach

F Ziaee, M Hajjari, SR Kazeminezhad, M Behmanesh
Journal Papers , , {Pages }

Abstract

Investigating the Cytotoxic Effect of Pinoresinol and Lariciresinol on Breast Cancer Cell Line SKBr3

M Soltani, N Ahmadian Chashmi, M Sharifi, R Fotovat, M Behmanesh
Journal PapersModares Journal of Biotechnology , Volume 11 , Issue 1, 2020 March 10, {Pages 13-20 }

Abstract

Aims: Breast cancer is the most common malignancy in women throughout the world. Among the various methods for preventing and treating cancer, plant natural compounds have more benefits than chemical drugs and have less side effects. Recently, many studies have been carried out on the antioxidant, anticancer, antiproliferation, and antiinflammatory properties of plant lignans, indicating the importance of these compounds in the prevention and treatment of various diseases. In the present study, the cytotoxic effects and inducing apoptosis of pinoresinol and lariciresinol lignans were investigated on SKBr3 breast cancer cell line.Materials & Methods: SKBr3 cells were treated with different concentrations of both pinoresinol and lariciresinol

Combined Inhibition of Menin-MLL Interaction and TGF-β Signaling Induces Replication of Human Pancreatic Beta Cells

Saghar Pahlavanneshan, Mehrdad Behmanesh, Daniel Oropeza, Kenichiro Furuyama, Yaser Tahamtani, Mohsen Basiri, Pedro L Herrera, Hossein Baharvand
Journal PapersEuropean Journal of Cell Biology , 2020 May 30, {Pages 151094 }

Abstract

Both type 1 and type 2 diabetes are associated with hyperglycemia and loss of functional beta cell mass. Inducing proliferation of preexisting beta cells is an approach to increase the numbers of beta cells. In this study, we examined a panel of selected small molecules for their proliferation-inducing effects on human pancreatic beta cells. Our results demonstrated that a small molecule inhibitor of the menin-MLL interaction (MI-2) and small molecule inhibitors of TGF-β signaling (SB431542, LY2157299, or LY364947) synergistically increased ex vivo replication of human beta cells. We showed that this increased proliferation did not affect insulin production, as a pivotal indication of beta cell function. We further provided evidence which

Drug-eluting PCL/graphene oxide nanocomposite scaffolds for enhanced osteogenic differentiation of mesenchymal stem cells

Fatemeh Rostami, Elnaz Tamjid, Mehrdad Behmanesh
Journal PapersMaterials Science and Engineering: C , 2020 May 20, {Pages 111102 }

Abstract

Recently, drug-eluting nanofibrous scaffolds have attracted a great attention to enhance the cell differentiation through biomimicking the extracellular matrix (ECM) in regenerative medicine. In this study, electrospun nanocomposite polycaprolactone (PCL)-based scaffolds containing synthesized graphene oxide (GO) nanosheets and osteogenic drugs, i.e. dexamethasone and simvastatin were fabricated. The physicochemical and surface properties of the scaffolds were investigated through FTIR, wettability, pH, and drug release studies. The cell viability, differentiation, and biomineralization were studied on mesenchymal stem cells (MSCs) by Alamar Blue, alkaline phosphatase (ALP) activity, and Alizarin Red-S staining, respectively. Uniformly dist

Association of Selenoprotein S Expression and its Variants with Metabolic Syndrome in Subjects with Cardiovascular Disease

Mojgan Gharipour, Mehrdad Behmanesh, Mansoor Salehi, Golnaz Vaseghi, Pouya Nezafati, Minoo Dianatkhah, Elham Khosravi, Mohsen Hosseini, Masoumeh Sadeghi
Journal PapersArchives of Medical Research , 2020 May 27, {Pages }

Abstract

BackgroundSelenoproteins S (SELS or VIMP) may regulate cytokine production, and thus play a key role in the control of the inflammatory response.MethodsThis study consisted of 136 Iranian patients with cardiovascular disease (65 MetS-affected and 71 MetS un-affected individuals) in the selengene study. Expression of two variants of VIMP including VIMP I and II were analyzed in all subjects using Real-Time PCR and ELISA.ResultsThe level of VIMP was lower in MetS+ compared to the MetS− subjects (p <0.05). We found no significant differences in quantitative expression of VIMP I and VIMP II in both groups. VIMP I reveal a reverse correlation with fasting blood sugar (FBS) (r?=?−0.45, p?=?0.009). Moreover, SELS in protein level has negative

Directed Blocking of TGF-β Receptor I Binding Site Using Tailored Peptide Segments to Inhibit its Signaling Pathway

Reza H Sajedi, Sepideh Sepehri, S Arab, Mehrdad Behmanesh
Journal PapersIranian Journal of Biotechnology , 2020 June 2, {Pages }

Abstract

Background: TGF-β isoforms play crucial roles in diverse cellular processes. Therefore, targeting and inhibiting TGF-β signaling pathway provides a potential therapeutic opportunity. TGF-β isoforms bind and bring the receptors (TβRII and TβRI) together to form a signaling complex in an ordered manner.Objectives: Herein, an antagonistic variant of TGF-β (AnTβ) has been designed and prepared to inhibit the formation of signaling complex and consequently its signaling pathway. This TGF-β homodimeric variant contains intact TβRII binding sites and blocked TβRI binding sites by substituting three peptide segments. So, AnTβ could only bind to TβRII, but prevent binding and recruitment of TβRI to form a signaling complex.Materials and

Comparative effects of nitric oxide and salicylic acid on salinity tolerance in saffron (Crocus sativus)

Samareh Babaei, Vahid Niknam, Mehrdad Behmanesh
Journal PapersPlant Biosystems-An International Journal Dealing with all Aspects of Plant Biology , 2020 March 3, {Pages 10-Jan }

Abstract

Salinity is a major environmental stress worldwide that adversely affects plant’s growth and metabolism. Various studies have demonstrated the positive effect of growth regulators on improving stress tolerance of plants. In this study, Crocus sativus as a profitable herb and expensive spice was subjected to nitric oxide and salicylic acid treatments to improve its sustainability under salinity stress. Based on our results treating Crocus sativus corms with nitric oxide caused more growth under salinity stress, also nitric oxide was able to reduce stress effects by compatible solutes accumulation, inducing antioxidative enzyme activities and increasing the biosynthesis of secondary metabolites. However, salicylic acid treatment did not imp

evaluation of Rationally Designed Label-free Stem-loop DnA probe Opening in the Presence of miR-21 by circular Dichroism and fluorescence techniques

Nasrin Farahani, Mehrdad Behmanesh, Bijan Ranjbar
Journal PapersScientific reports , Volume 10 , Issue 1, 2020 March 4, {Pages 11-Jan }

Abstract

The characteristic features of stem-loop structured probes make them robust tools to detect targets with high sensitivity and selectivity. The basis of the hairpin based sensors operation is a conformational change that occurs upon hybridization of target with stem-loop probe. The design of the stem-loop probe has an important role in target recognition. Therefore, we designed a label-free stem loop probe for targeting miR-21 as a cancer biomarker investigated by web-based tools; its thermodynamic parameters obtained by thermal UV spectroscopy. The efficiency of stem-loop structure opening in the presence of target and non-target sequences was evaluated by fluorescence spectroscopy and circular dichroism spectro-polarimetry. The results sho

Hsa-miR-587 Regulates TGFβ/SMAD Signaling and Promotes Cell Cycle Progression

Mahnaz Jahangirimoez, Abdallah Medlej, Mahmoud Tavallaie, Bahram Mohammad Soltani, Sohameh Mohebbi, Tahereh Tohidi Moghadam, Maryam Nikkhah, Mehrdad Behmanesh, Morvarid Saeinasab, Ahmad Reza Bahrami, Jovanna Gonz?lez, Maite Huarte, Maryam Hassanlou, Bahra
Journal Paperspathways , Volume 12 , 2020 July 1, {Pages 16 }

Abstract

Objective: Transforming growth factor beta/single mothers against decapentaplegic (TGFβ/SMAD) signaling pathway plays important roles in various biological processes. It acts as a tumor suppressor during the early stages of cancer progression. Discovering the regulators of this pathway provides important options for therapeutic strategies. Here, we searched for candidate microRNAs (miRNAs) that potentially target the critical components of the TGFβ signaling pathway.Materials and Methods: In the current experimental study, we first predicted miRNAs that target TGFβ components using a bioinformatics software. After that, quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-587, TGFBR2, SMAD4,

The probable role of GPR182 and CALCRL genes in endothelial dysfunction caused by hyperglycemia

Mehrdad Behmanesh
Journal PapersModares Journal of Biotechnology , Volume 11 , Issue 2, 2020 June 10, {Pages 249-256 }

Abstract

Hyperglycemia is a major cause of diabetes. Hyperglycemia-induced endothelial dysfunction is generally believed to be the basis of diabetic vascular complications such as retinopathy, nephropathy and cardiovascular diseases. The most important molecules in endothelial cells that can sense elevated level of glucose and transmit signals into the cell are G protein-coupled receptors (GPCRs).In the present study, according to bioinformatics analysis of genomic sequences between healthy and patient individuals, two G proteins GPR182 and CALCRL were selected and their expression level were examined in hyperglycemic and normal conditions in HUVEC as a model of vascular endothelial cells at different glucose concentrations and various time interval

The effect of chemokine CXCR3 receptor downregulation on vascular endothelial cell dysfunction

Seyedeh Fatemeh Sajjadi, Mehrdad Behmanesh
Journal PapersModares Journal of Biotechnology , Volume 11 , Issue 3, 2020 October 10, {Pages 0-0 }

Abstract

Atherosclerosis is a chronic vascular disease and remains the leading cause of death and morbidity worldwide. Endothelial dysfunction is an important factor in the progression of atherosclerotic disease. Increased expression of cell adhesion index genes and decreased cell-binding proteins lead to abnormal endothelial function. These molecular changes are one of the most important indicators of endothelial cell dysfunction and the progression of atherosclerosis. CXCR3 is a G-protein-coupled chemokine receptor expressed by endothelial cells. The role of the receptor CXCR3 and its ligands in endothelial cells and heart disease is not yet fully understood. In this study, we evaluated the effect of CXCR3 downregulation on the expression level of

Evaluation of microRNA Gene Polymorphisms in Liver Transplant Patients with Hepatocellular Carcinoma

Sahar Dehbidi, Zhaleh Farokhizadeh, Mohammad Hossein Karimi, Afsoon Afshari, Mehrdad Behmanesh, Mohammad Hossein Sanati, Bita Geramizadeh, Ramin Yaghobi
Journal PapersHepatitis Monthly , Volume 20 , Issue 7, 2020 July 31, {Pages }

Abstract

Background: Genetic polymorphism in the miRNA sequence might alter miRNA expression and/or maturation, which is associated with the development and progression of hepatocellular carcinoma (HCC) in liver transplant patients. Objectives: Therefore, the prevalence of miRNA-146a G > C (rs2910164), miRNA-499A > G (rs3746444), miRNA-149C > T (rs2292832), and miRNA-196a-2 C > T (rs11614913) gene polymorphisms was evaluated in liver recipients with HCC with or without experiencing graft rejection. Methods: In a cross-sectional study, tissue samples were collected from 60 HCC patients who underwent liver transplant surgery at Namazi Hospital, Shiraz, Iran, in 2013 - 2015. A control group consisting of 120 individuals was randomly selected, as well.

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