The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus
Ameneh Shokati, Masoud Soleimani, Saeid Abroun
Journal Papers , 2021 January 27, {Pages }
Abstract
Background: The epidemiological studies indicated that colorectal cancer is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus supernatant (LAS) and lactobacillus rhamnosus supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro.Methods: In this experimental study, the anti-proliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chambers assays, respectively. The expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR.Resu
Mesenchymal stem cell‐derived extracellular vesicles conditionally ameliorate bone marrow failure symptoms in an immune‐mediated aplastic anemia mouse model
Mohammad A Gholampour, Saeid Abroun, Rienk Nieuwland, Seyed J Mowla, Sara Soudi
Journal PapersJournal of cellular physiology , 2021 January 25, {Pages }
Abstract
Acquired forms of Aplastic anemia (AA) are characterized by T cell‐mediated immune disease resulting in bone marrow (BM) failure and marrow hypoplasia. In these cases, it is a major challenge to modulate autoreactive T cell activity and thereby decrease the pro‐inflammatory cytokine storm. Emerging evidence indicates that extracellular vesicles derived from mesenchymal stem cells (MSC‐EVs) control and modulate immunity. The therapeutic potential of MSC‐EVs has not been investigated in acquired AA. Hence, in this study, we constructed an AA mice model through irradiation and splenocyte infusion to test the benefits of hypoxic MSC‐EVs (Hx‐EVs) and normoxic MSC‐EVs (Nx‐EVs). We found that MSC‐EVs treatment significantly prol
Breast Cancer Cell Lines, HER2/Neu Phenotype, and a Higher Propensity to Reactive Oxygen Species Production
Zahra Mohammadi Abgarmi, Abbas Sahebghadam Lotfi, Saeid Abroun, Masoud Soleimani, Shahla Mohammad Ganji, Parvaneh Baktash, Arash Moradi
Journal PapersArchives of Breast Cancer , 2021 April 15, {Pages 137-142 }
Abstract
Background: The reactive oxygen species (ROS) generated in the biological systems play an important role in pathological conditions and specific normal cellular processes, such as signaling pathways and drug sensitivity.Methods: Intracellular ROS was determined using (2'-7'dichlorofluorescin diacetate) DCFH-DA fluorimetric probe, malondialdehyde as lipid peroxidation index detected by the Thiobarbituric acid reactive substances method. Cells' protein carbonyl contents were assessed with 2, 4-Dinitrophenylhydrazine (DNPH) derivatization spectrophotometrically at 360-385 nm.Results: The oxidative stress induces ROS (p? 0.05), MDA (Malondialdehyde)(p? 0.05), and protein carbonylation (p? 0.01) was significantly higher in HER2-positive BT-474,
Objective: Multiple myeloma (MM) is an incurable plasma cell malignancy. Several genetic and epigenetic changes affect numerous critical genes expression status in this disorder. CDKN2A gene is expressed at low level in almost all cases with MM disease. The mechanism of this gene down-regulation has remained controversial. In the present study, we targeted EZH2 by microRNA-124 (miR-124) in L-363 cells and assessed following possible impact on CDKN2A gene expression and phenotypic changes.Materials and Methods: In this experimental study, growth inhibitory effects of miR-124 were measured by MTT assay in L-363 cell line. Likewise, cell cycle assay was measured by flowcytometery. The expression levels of EZH2 and CDKN2A were evaluated by real
Evaluation of stemness genes expression of OCT4, SOX2, Nanog, C-Myc and surface marker of CD133 on myeloma cells
Saeid Abroun, Masoud Soleimani
Journal PapersJournal of Research in Applied and Basic Medical Sciences , Volume 6 , Issue 2, 2020 April 10, {Pages 96-103 }
Abstract
Cancer stem cells that have the ability to proliferation and self-renewal were cause to drug resistance and metastasis in malignancies. In multiple myeloma, these cancer stem cells (MMCSCs) play a very important role in the recurrence of the disease. Detection of these cells can create new ways for treating these patients. In this study, stemness genes and phenotypic markers were investigated as the methods for identifying cancer stem cells in multiple myeloma. Expression of stemness genes of OCT4, SOX2, Nanog, C-Myc and target genes of STAT3 and TCF3 by PCR and Real Time PCR and also expression of surface markers of CD19, CD33, CD133, CD38 and CD56 by flow cytometry in RPMI8226 and U266 were studied. The results of the study showed that my
Although numerous replication case-control studies have attempted to determine the association between Factor V Leiden (FVL) 1691G > A mutation and susceptibility to Recurrent pregnancy loss (RPL), there have been confliction among the results of various ethnic groups. To address this limitation, here we implemented first meta-analysis to provide with consistent conclusion of the association between FVL 1691G > A mutation and RPL risk. After a systematic literature search, pooled odds ratio (OR) and their corresponding 95% confidence interval (CI) were used to evaluate the strength of the association. Additionally, meta-regression analyses were performed to find potential source of heterogeneity. In this meta-analysis, 62 studies, c
Severe combined immunodeficiency (SCID) is classified as a primary immunodeficiency, which is characterized by impaired T-lymphocytes differentiation. IL2RG, IL7Ralpha, JAK3, ADA, RAG1/RAG2, and DCLE1C (Artemis) are the most defective genes in SCID. The most recent SCID therapies are based on gene therapy (GT) of hematopoietic stem cells (HSC), which are faced with many challenges. The new studies in the field of stem cells have made great progress in overcoming the challenges ahead. In 2006, Yamanaka et al. achieved" reprogramming" technology by introducing four transcription factors known as Yamanaka factors, which generate induced pluripotent stem cells (iPSC) from somatic cells. It is possible to apply iPSC-derived HSC for transplantati
Plasma Cell Proliferation Is Reduced in Myeloma-Induced Hypercalcemia and in Co-Culture with Normal Healthy BM-MSCs
Nader Vazifeh Shiran, Saeid Abroun
Journal PapersLaboratory Medicine , 2020 October 14, {Pages }
Abstract
Objective In multiple myeloma (MM), stimulation of osteoclasts and bone marrow (BM) lesions lead to hypercalcemia, renal failure, and anemia. Co-culture of the myeloma cells in both hypocalcemia and hypercalcemia concentrations with bone marrow-mesenchymal stem cells were evaluated. Materials and Methods Viability and survival of myeloma cells were assessed by microculture tetrazolium test and flow cytometric assays. Mesenchymal stem cells (MSCs) were extracted from normal and myeloma patients and were co-cultured with myeloma cells. Results Myeloma cells showed less survival in both hypocalcaemia and hypercalcemia conditions (P <.01)
Stem Cell Therapy: A Promising Approach in Treatment of COVID 19.
Bahareh Abbaspanah, Saeid Abroun, Morteza Zarrabi, Ashkan Mozdgir, Mohammad Mollanouri
Journal Papers , 2020 October 12, {Pages }
Abstract
COVID-19 pandemic is a global health crisis in the 21st Century. There are currently no approved vaccine and no particular antiviral treatment for coronavirus disease. As COVID-19 has had a broad range of illnesses, it is necessary to find a safe and effective therapeutic method for COVID-19. An attractive approach for treating COVID-19 is cell therapy. Cell therapy aims to inject new and healthy stem cells into a patient's body, to repair the damaged cells and tissues. Stem cell therapy is one of the most studied and important approaches in treatment of COVID-19 these days. The significant clinical outcome was observed by the adoptive transfer of stem cells, specifically mesenchymal stem cells. This study reviews the characteristics of ste
Inhibition of miR-19a-3p Increases Bortezomib-Induced Apoptosis in Myeloma Cell Lines by Targeting SOCS3
Azam KAZEMI, Saeid ABROUN, Masoud SOLEIMANI
Journal PapersTURKISH JOURNAL OF ONCOLOGY , Volume 1 , Issue 3, 2020 January , {Pages }
Abstract
OBJECTIVEApoptosis is considered the main factor inducing by bortezomib in myeloma cells. Although bortezomib (BTZ) is a crucial drug for the treatment of MM, chemoresistance is a major problem. OncomiR-19a plays an oncogenic role in many types of cancer such as MM; however, the function of miR-19a in the pathogenesis of MM and drug resistance has not been identified well. The present research aims at investigating the inhibition of miR-19a by antagomir to conclude BTZ responsiveness and suggests that miR-19a may be a biomarker for the prognosis of the patient.
Microvesicles (MVs) are the smallest subclass of the extracellular vesicles (EVs) spontaneously secreted by the external budding from the cell membranes in physiologic and pathologic conditions. The MVs derived from leukemic cells (LCs) can be detected by the expression of specific cluster of differentiation (CD) markers indicating their cellular origin while they can transfer different agents such as microRNAs, cytokines, and chemokines. The secretion of these agents from MVs can affect the vital processes of LCs such as cell cycle, proliferation, differentiation, and apoptosis. According to the effects of MVs components on the vital processes of LCs, it has been postulated that a change in the expression of MVs might be involved in the pr
Comparison of cord blood CD34+ stem cell expansion in coculture with mesenchymal stem cells overexpressing SDF‐1 and soluble/membrane isoforms of SCF
Mansoureh Ajami, Masoud Soleimani, Saeid Abroun, Amir Atashi
Journal PapersJournal of cellular biochemistry , 2019 May 17, {Pages }
Abstract
Aim Umbilical cord blood hematopoietic stem cells (UCB HSCs) have been considered for the treatment of hematological malignancies due to their noninvasive collection, greater capacity of expansion, and remarkable tolerance for HLA mismatch in transplantation. On the other hand, the most considerable limitation of these cells is their inadequate amount of CD34 + HSCs which leads to delayed engraftment. The aim of this study was the expansion of CD34 + HSCs by coculturing with mesenchymal stem cells (MSCs) overexpressing stromal cell‐derived factor‐1, soluble and membrane isoforms of stem cell factor (sSCF/mSCF). Keeping structure and function of overexpressed cytokines by MSCs was expected which could beneficially affect the CD
Amniotic fluid, an effective factor for umbilical cord blood hematopoietic stem cells in cell culture: An approach for bone marrow transplantation
Tayebe Torabi, Saeid Abroun
Journal PapersTransfusion and Apheresis Science , Volume 58 , Issue 2, 2019 April 1, {Pages 169-173 }
Abstract
Bone marrow transplantation is a treatment used for hematologic and non-hematologic disorders. A theory suggests that proliferation of cells in non-body condition helps to increase the efficiency of bone marrow transplant. There are different ways for proliferation of stem cells, in which, most studies have focused on stem cell culture in body-like conditions. The use of amniotic fluid as a rich resource of growth factors is developing in repair of tissues cornea. With regards to this condition, we discuss about the influence of amniotic fluid in proliferation and implantation of blood stem cells. The aim of this study was investigation of human amnion fluid (HAF) in support of growth and proliferation of umbilical cord in order to transpla
Journal PapersInternational Journal of Medical Laboratory , 2019 September 2, {Pages }
Abstract
Background and Aims: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are a well-known source of multipotent adult stem cells. Despite using different methodologies of MSCs preparing for clinical applications, the top safest procedure to manipulate these cells, has not yet been determined. Recently, ex-vivo expansion of MSCs for their subsequent implantation, using some biological product, is suggested instead of fetal bovine serum (FBS). Previous studies have shown the effect of follicular fluid (FF)(a dynamic fluid in ovarian follicle) as an additive component in cell culture. Hence, this study aimed to decipher its role on the human BM-MSC proliferation.Materials and Methods: In this study, BM-MSCs at 3 rd passage were cultivated in
Potential of Polycaprolactone Nanofi-ber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells
SH Mousavi, S Abroun, M Soleimani, SJ Mowla
Journal PapersInt J Stem Cell Res Ther , Volume 5 , 2019 January , {Pages 059 }
Abstract
Background: An efficient and practical ex vivo expansion of cord blood hematopoietic stem cells as an alternative source of HSC transplantation is crucial in understanding the potential of HSC transplantation in treating or supportive therapy in a variety of hematologic and non-hematologic disorders. The aim of this study was an ex vivo expansion of cord blood hematopoietic stem cells in a novel threedimensional polycaprolactone nanofiber scaffold coated with collagen.Methods: After 10-day cultured of cord blood CD34+ cells in 2-Dimensional and 3-Dimensional culture system, the evaluation was performed by qRT-PCR, flow cytometry and clonogenicity.Results: 3-Dimensional Polycaprolactone nano-scaffold coated with collagen provided higher tota
Background: The disease-associated cell lines used in the majority of preclinical trials should be first authenticated and identified to avoid directing research expenditure on wrong cells and obtaining irrelevant results. The present study evaluated the immunophenotypic and immunotypic authenticity of six human myeloma cell lines (HMCLs).Materials and Methods: Cytospin smear and Wright staining were used for invetigating cell morphology, the flowcytometry of the markers CD45, CD2, CD19, CD38 and CD138 for immunophenotypic investigations, capillary electrophoresis for the immunoglobulin secretion potency and immunofixation for the immunotypical investigation of the cells.Results: Given the definite plasma cell morphology, the identity of al
Journal PapersAsian Journal of Transfusion Science , Volume 13 , Issue 2, 2019 July , {Pages 79 }
Abstract
Umbilical cord blood (UCB) is an attractive source of hematopoietic stem cells for transplantation in some blood disorders. One of the major factors that influence on transplantation fate is cord blood (CB) cell count, in addition to human leukocyte antigen similarity and CD34+ cell number. Here, we review the factors that could effect on quality and quantity of CBUs. Relevant English-language literatures were searched and retrieved from PubMed using the terms: CB, quality, collection, and transplantation. The numbers of total nucleated cells (TNCs) and CD34+ cells are good indicators of CB quality because they have been associated with engraftment; thereby, whatever the TNCs in a CB unit (CBU) are higher, more likely they led to successful
The effect of human amniotic fluid on the survival and proliferation of human myeloma cell lines RPMI8226 and U266 in comparison with the fetal bovine serum
Fatemeh Mojtahedi, Reza Mansouri, Saeed Abroun
Journal PapersRazi Journal of Medical Sciences , Volume 26 , Issue 4, 2019 July 1, {Pages 54-69 }
Abstract
Background: Multiple Myeloma is a Plasma Cell Malignancy. Since the study of pathogenicity mechanisms and messenger pathways involved in the causative agent cells is important in the laboratory environment and close to the physiological environment of the body, therefore, the best environment for the study of cells in the laboratory environment, an environment most closely resembling the physiological environment of the body. However, the use of fetal bovine serum (FBS) as a supplement to the growth of cells in the cell culture process is very common. Therefore, the aim of this study was to investigate the effect of amniotic fluid on proliferation (Ki-67 and Cyclin-D) and survival (Bax and Bcl2) of both RPMI8226 and U266 Myeloma in comparis
بررسي امكان پيش بيني وقوع بيماري پيوند عليه ميزبان مزمن(cGVHD) در كودكان پس از پيوند آلوژن سلول هاي بنيادي خونساز با مطالعه پروفايل بيان ژن سلول هاي ايمني زيرگروه هاي T و NKcell
1398
طاري, كاوه
القاي لوسمي لنفوبلاستي حاد T ( (T-ALL انساني در مدل موشي NOD و بررسي تاثير داروي شيمي درماني ونتوكلاكس(Venetoclax) بر بيان ژن هاي خانواده BCL-2 مسير آپوپتوز(پيش آپوپتوزي و مهار كننده آپوپتوز)
1398
قاسمي مهر, نرگس
ارزيابي اوليه تزريق سلول هاي كشنده طبيعي از اهداكنندگان آلوژن به ظاهر سالم در بيماران مبتلا به مالتيپل مايلوماي پرخطر
1399
جديدطواف, مريم
نتيجه تيمار با Platelet-rich plasma (PRP) مشتق از خون بند ناف بر روي خواص فيبروژنيك (HSC) Hepatic Stellate Cells