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  • دکتری (1389)

    اصلاح نباتات - ژنتیک مولکولی ومهندسی ژنتیک

    دانشگاه تبریز، تبریز، ایران

  • کارشناسی‌ارشد (1383)

    اصلاح نباتات

    دانشگاه تبریز، تبریز، ایران

  • مهندسی ژنتیک متابولیت‌های ثانویه
  • مهندسی ژنتیک مقاومت به تنش‌های زیستی مهندسی ژنتیک تحمل به تنش‌های غیر‌زیستی

    دکتر محمدصادق ثابت درجه کارشناسی را در رشته زراعت و اصلاح‌نباتات در سال 1381، درجه کارشناسی‌ارشد اصلاح‌نباتات در سال 1383 از دانشگاه تبریز و دکتری اصلاح نباتات را با گرایش ژنتیک مولکولی و مهندسی ژنتیک در سال 1389 از دانشگاه تبریز کسب نمود. او در آذرماه سال 1390 به هیئت علمی دانشگاه تربیت مدرس تهران پیوست که هم اکنون استادیار گروه ژنتیک و به‌نژادی گیاهی در دانشکده کشاورزی است. زمینه‌های تحقیقاتی ایشان شامل: مهندسی متابولیک در گیاهان دارویی، مطالعه پروفایل بیانی ژن های مهم مسیر بیوسنتزی متابولیت های ثانویه دارویی، اصلاح مولکولی مقاومت به تنشهای زیستی و غیرزیستی به روش Gene Silencing و Gene Overexpression می‎باشد.

    ارتباط

    رزومه

    Relationship between expression pattern of phosphate transporter genes and the phosphatase enzyme PAP26 in response to stress phosphorus deficiency in Arabidopsis thaliana

    Mohammad Sadegh Sabet, Mohaddeseh Zal, Mohammad Ali Malboobi
    Journal Papersفصلنامه علمی ژنتیک نوین , Volume 16 , Issue 1, 2021 March 10, {Pages 63-72 }

    Abstract

    Phosphorus (P) is one of the most vital elements for all living organisms, which acts as a constituent of essential biomolecules and metabolic reactions. Plants to cope with phosphate (Pi) deficiency have developed different adaptive mechanisms, including induction of acid phosphatases genes, Pi transporters and release of organic acids in rhizosphere as well. Under conditions of phosphorus deficiency stress, maintaining cellular phosphorus homeostasis depends on the interactions between these components. In the present study, the relationship between gene expression pattern of three families of phosphate transporters PHT1, PHT2 and PHO1 in Arabidopsis thaliana in interaction with AtPAP26 as the dominant purple acid phosphatase among 29 ide

    Identification and Functional Analysis of Two Purple Acid Phosphatases AtPAP17 and AtPAP26 Involved in Salt Tolerance in Arabidopsis thaliana Plant

    Mohammad Ali Abbasi-Vineh, Mohammad Sadegh Sabet, Ghasem Karimzadeh
    Journal PapersFrontiers in Plant Science , Volume 11 , 2021 February 15, {Pages 2326 }

    Abstract

    Tolerance to salinity is a complex genetic trait including numerous physiological processes, such as metabolic pathways and gene networks; thereby, identification of genes indirectly affecting, as well as those directly influencing, is of utmost importance. In this study, we identified and elucidated the functional characterization of AtPAP17 and AtPAP26 genes, as two novel purple acid phosphatases associated with high-salt tolerance in NaCl-stressed conditions. Here, the overexpression of both genes enhanced the expression level of AtSOS1, AtSOS2, AtSOS3, AtHKT1, AtVPV1, and AtNHX1 genes, involving in the K+/Na+ homeostasis pathway. The improved expression of the genes led to facilitating intracellular Na+ homeostasis and decreasing the io

    Bottleneck Removal of Paclitaxel Biosynthetic Pathway By Overexpression of DBTNBT Gene Under Methyl-β-Cyclodextrin and Coronatine Elicitation in Taxus Baccata L.

    K Kashani, MS Sabet, MJ Javaran, A Moieni
    Journal Papers , , {Pages }

    Abstract

    Distribution of Zinc in Bread Wheat Genotypes with High and Low Zn-content

    S Fathi, MS Sabet, T Lohrasebi
    Journal Papers , , {Pages }

    Abstract

    Effect of AtPAP17 and AtPAP26 genes overexpression on yield and yield components under salt stress in Arabidopsis thaliana plant

    MA Abbasi Vineh, MS Sabet, G Karimzadeh
    Journal Papers , , {Pages }

    Abstract

    The expression patterns of phosphate transporter genes in relationship with PAP26 encoding a phosphatase enzyme in Arabidopsis thaliana

    M Zal, MS Sabet, MA MALBOOBI
    Journal Papers , , {Pages }

    Abstract

    Heterologous expression of an acid phosphatase gene and phosphate limitation leads to substantial production of chicoric acid in Echinacea purpurea transgenic?…

    Meisam Salmanzadeh, Mohammad Sadegh Sabet, Ahmad Moieni, Mehdi Homaee
    Journal PapersPlanta , Volume 251 , Issue 1, 2020 January 1, {Pages 31 }

    Abstract

    Main conclusion A high level of the secondary metabolite chicoric acid is produced by intracellular Pi supply and extracellular phosphate limiting in Echinacea purpurea hairy roots. Abstract Chicoric acid (CA) is a secondary metabolite which is gained from Echinacea purpurea. It has been found to be one of the most potent HIV integrase inhibitors with antioxidant and anti-inflammatory activities. However, the low-biosynthesis level of this valuable compound becomes an inevitable obstacle limiting further commercialization. Environmental stresses, such as phosphorus (Pi) deficiency, stimulate the synthesis of chemical metabolites, bu

    Zinc and potassium fertilizer recommendation for cotton seedlings under salinity stress based on gas exchange and chlorophyll fluorescence responses

    Zahra Hatam, Mohammad Sadegh Sabet, Mohammad Jafar Malakouti, Ali Mokhtassi-Bidgoli, Mehdi Homaee
    Journal PapersSouth African Journal of Botany , Volume 130 , 2020 May 1, {Pages 155-164 }

    Abstract

    Cotton is typically grown in warm regions in which salinity and nutrient deficiency stresses are ubiquitous and often simultaneously influence plant growth. Under saline conditions, fertilizer recommendation is highly challenging, since nutrient application may increase or decrease plant salt tolerance, which may complicate prediction of crop yield. So far, no investigations have been conducted in salt-affected soils to determine optimum concentrations of potassium (K) and zinc (Zn) fertilizers based on chlorophyll fluorescence (ChlF) and gas exchange (GEx) responses in upland cotton (Gossypium hirsutum L.). Accordingly, in this study, a factorial experiment was conducted in a complete block design with six replicates under controlled condi

    Fungal Cell Wall and Methyl-β–Cyclodextrin Synergistically Enhance Paclitaxel Biosynthesis and Secretion in Corylus avellana Cell Suspension Culture

    Farhadi Siamak, Moieni Ahmad, Safaie Naser, Mohammad Sadegh Sabet, Salehi Mina
    Journal PapersScientific Reports (Nature Publisher Group) , Volume 10 , Issue 1, 2020 December 1, {Pages }

    Abstract

    Paclitaxel is the top-selling chemotherapeutic drug used for the treatment of lung, ovarian and breast cancer as well as Kaposi’s sarcoma. Cell suspension culture (CSC) of Corylus avellana has been addressed as a promising alternative for producing paclitaxel. In this study, endophytic fungus strain YEF 33 was isolated from Taxus baccata and identified as Coniothyrium palmarum. The effects of the elicitors derived from this fungus including cell extract, culture filtrate and cell wall (CW) and also chitin, alone or in combination with Methyl-β-Cyclodextrin (MBCD), on paclitaxel biosynthesis in C. avellana CSC were assayed for the first time. CW of C. palmarum was the most efficient fungal elicitor for paclitaxel biosynthesis in C. avella

    fungal cell Wall and Methyl-β–cyclodextrin Synergistically enhance paclitaxel Biosynthesis and Secretion in Corylus avellana cell Suspension culture

    Siamak Farhadi, Ahmad Moieni, Naser Safaie, Mohammad Sadegh Sabet, Mina Salehi
    Journal PapersScientific reports , Volume 10 , Issue 1, 2020 March 25, {Pages 10-Jan }

    Abstract

    Paclitaxel is the top-selling chemotherapeutic drug used for the treatment of lung, ovarian and breast cancer as well as Kaposi’s sarcoma. Cell suspension culture (CSC) of Corylus avellana has been addressed as a promising alternative for producing paclitaxel. In this study, endophytic fungus strain YEF 33 was isolated from Taxus baccata and identified as Coniothyrium palmarum. The effects of the elicitors derived from this fungus including cell extract, culture filtrate and cell wall (CW) and also chitin, alone or in combination with Methyl-β-Cyclodextrin (MBCD), on paclitaxel biosynthesis in C. avellana CSC were assayed for the first time. CW of C. palmarum was the most efficient fungal elicitor for paclitaxel biosynthesis in C. avella

    Modeling of paclitaxel biosynthesis elicitation in Corylus avellana cell culture using adaptive neuro-fuzzy inference system-genetic algorithm (ANFIS-GA) and?…

    Siamak Farhadi, Mina Salehi, Ahmad Moieni, Naser Safaie, Mohammad Sadegh Sabet
    Journal PapersPlos one , Volume 15 , Issue 8, 2020 August 27, {Pages e0237478 }

    Abstract

    Paclitaxel as a microtubule-stabilizing agent is widely used for the treatment of a vast range of cancers. Corylus avellana cell suspension culture (CSC) is a promising strategy for paclitaxel production. Elicitation of paclitaxel biosynthesis pathway is a key approach for improving its production in cell culture. However, optimization of this process is time-consuming and costly. Modeling of paclitaxel elicitation process can be helpful to predict the optimal condition for its high production in cell culture. The objective of this study was modeling and forecasting paclitaxel biosynthesis in C. avellana cell culture responding cell extract (CE), culture filtrate (CF) and cell wall (CW) derived from endophytic fungus, either individually or

    The Critical Role of AtPAP17 and AtPAP26 Genes in Arabidopsis Phosphate Compensation Network

    Chilak Siamak, Mohammad Sadegh Sabet, Mohammad ALi Malboobi, Ahmad Moieni
    Journal PapersFrontiers in plant science , Volume 11 , 2020 January , {Pages 1495 }

    Abstract

    Purple acid phosphatases (PAP)-encoding genes form a complex network that play a critical role in plant phosphate (Pi) homeostasis. Mostly, the functions of PAPs were investigated individually. However, the interactions of most of these genes in response to various concentrations of available Pi remain unknown. In this study, the roles of AtPAP17 and AtPAP26 genes, and their relationship within Pi homeostasis context were investigated. Surprisingly, atpap17 and atpap26 mutants not only showed no obvious developmental defects, but also produced higher biomass in compare to wild type (WT) plants under normal growth conditions. Comparing gene expression patterns of these mutants with WT plant, we identified a set of genes up-regulated in mutan

    Identification of a defense response gene involved in signaling pathways against PVA and PVY in potato

    Zhila Osmani, Mohammad Sadegh Sabet, Kenji S Nakahara, Ali Mokhtassi-Bidgoli, Khabat Vahabi, Ahmad Moieni, Masoud Shams-Bakhsh
    Journal PapersGM Crops & Food , Volume 12 , Issue 1, 2020 October 7, {Pages 86-105 }

    Abstract

    Potato is the most important non-grain food crop in the world. Viruses, particularly potato virus Y (PVY) and potato virus A (PVA), are among the major agricultural pathogens causing severe reduction in potato yield and quality worldwide. Virus infection induces host factors to interfere with its infection cycle. Evaluation of these factors facilitates the development of intrinsic resistance to plant viruses. In this study, a small G-protein as one of the critical signaling factors was evaluated in plant response to PVY and PVA to enhance resistance. For this purpose, the gene expression dataset of G-proteins in potato plant under five biotic (viruses, bacteria, fungi, nematodes, and insects) and four abiotic (cold, heat, salin

    Effect of P19 gene expression on transcription rate and production of recombinant human tissue plasminogen (rtPA) in tobacco (nicotiana benthamiana)

    YOUSEF Sharafi, M Jalali Javaran, MOHAMMAD SADEGH Sabet
    Journal PapersAgricultural Biotechnology Journal , Volume 12 , Issue 2, 2020 January 1, {Pages 103-128 }

    Abstract

    ResultsThe results of Real Time PCR test showed that rtPA transcript increased in the presence of P19. Also, 4 days after plant inoculation, the highest transcript levels were obtained from p19 and rtPA genes. ELISA results showed that the expression of rtPA protein in the presence of P19 was 89 and 84 ?g. g-1 leaf weight at the 7 and 10 day after inoculation, respectively. This expression was 12 and 15% higher than of when agrobacterium-containing pCAMBIA1304-rtPA vector alone was used, respectively.

    Modeling of paclitaxel biosynthesis elicitation in Corylus avellana cell culture using adaptive neuro-fuzzy inference system-genetic algorithm (ANFIS-GA) and …

    S Farhadi, M Salehi, A Moieni, N Safaie, MS Sabet
    Journal Papers , , {Pages }

    Abstract

    Effect of Two Phosphatase AtPAP17 and AtPAP26 Gene Knockout on Growth Period Duration and Flowering in Arabidopsis thaliana

    S Farhadi, MS Sabet, A Moieni, MA Malboobi
    Journal Papers , , {Pages }

    Abstract

    Heterologous expression of an acid phosphatase gene and phosphate limitation leads to substantial production of chicoric acid in Echinacea purpurea transgenic hairy roots

    M Salmanzadeh, MS Sabet, A Moieni, M Homaee
    Journal Papers , , {Pages }

    Abstract

    Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots

    M Jafarzade, M Ramezani, F Hedayati, Z Mokhtarzade, B Zare, MS Sabet, P Norouzi, MA Malboobi
    Journal PapersThe Plant Pathology Journal , Volume 35 , Issue 6, 2019 December , {Pages 692 }

    Abstract

    Agrobacterium rhizogenes–mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided a

    A quantitative approach for fertilizer recommendation under saline conditions

    Zahra Hatam, Mohammad Sadegh Sabet, Mohmmad Jafar Malakouti, Mehdi Homaee
    Journal PapersArchives of Agronomy and Soil Science , Issue just-accepted, 2019 May 27, {Pages }

    Abstract

    All existing fertility models are originally derived for non-saline conditions. Under salinity, fertilization is challenging and may lead to amelioration or deterioration of plant salt tolerance. This investigation aimed to develop new deterministic models for fertilizer recommendation in salt-affected soils integrating salinity and fertility models. Based on statistical parameters, the nutritional-salinity nonlinear-nonlinear model was the most efficient followed by the linear-nonlinear model. The latter is preferred due to its accessible inputs. Model parameters were expressed as functions of nutrient rates and demonstrated drastic variations under fertilization. Zinc deficiency was more limiting than salinity while salinity was more detr

    Virus‐specific and common transcriptomic responses of potato (Solanum tuberosum) against PVY, PVA and PLRV using microarray meta‐analysis

    Zhila Osmani, Mohammad Sadegh Sabet, Masoud Shams‐Bakhsh, Ahmad Moieni, Khabat Vahabi
    Journal PapersPlant Breeding , Volume 138 , Issue 2, 2019 April , {Pages 216-228 }

    Abstract

    Potato as an essential root crop plays a fundamental role in sustenance security of the world. Viruses are among the most critical pathogens that can decrease the quality of products such as potato and their potential yield. Understanding plant responses to various stresses is an economically important subject in global food security, which is seriously threatened by climate changes. One of the effective methodologies to expand the resistance of potato against various stresses can be identified as the natural defence‐related genes. Microarray meta‐analysis was used for finding common patterns in microarray data sets. The results were filtered out by comparing the resistant and susceptible host data sets in combination with the data der

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    دروس نیمسال جاری

    • دكتري
      مهندسي ژنتيك پيشرفته ( واحد)
      دانشکده کشاورزی، گروه ژنتيك و به نژادي گياهي
    • كارشناسي ارشد
      مهندسي ژنتيك ( واحد)
    • كارشناسي ارشد
      مهندسي ژنتيك ( واحد)

    دروس نیمسال قبل

    • دكتري
      بيوانفورماتيك كاربردي ( واحد)
      دانشکده کشاورزی، گروه ژنتيك و به نژادي گياهي
    • دكتري
      بيوانفورماتيك كاربردي ( واحد)
    • كارشناسي ارشد
      ژنتيك پيشرفته ( واحد)
    • كارشناسي ارشد
      مباحث نوين در به نژادي گياهي ( واحد)
    • دكتري
      به نژادي گياهان براي تحمل به تنش هاي غير زيستي ( واحد)
    • دكتري
      ژنتيك ملكولي پيشرفته ( واحد)
    • 1396
      سوري, مجيد
      تاثير نوع هورمون اكسين و پيش سازهاي آن بر الگوي بياني ژن هاي موثر در غالبيت انتهايي در زعفران
    • 1400
      فتحي شيخي, مهسا
    • 1395
      شيرازي پارسا, هادي
      مهندسي مقاومت به برخي ويروس¬هاي خانواده Potyviridae با ايجاد تغيير در ژن eIF4E گياه خربزه inodorus Cucumis melo var.) ) به روش CRISPR/Cas9
    • 1396
      بزرگمهر, ابوالفضل
    • 1398
      دادگر, پريسا
    • 1399
      كريم بيگي, هادي
    • مدیر گروه اصلاح نباتات
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